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Table 1 Activity of enriched enzyme fraction with different electron donors

From: The respiratory molybdo-selenoprotein formate dehydrogenases of Escherichia coli have hydrogen: benzyl viologen oxidoreductase activity

Electron donor and acceptora Specific Activity
(mU mg protein-1)b
H2 and benzyl viologen 14.8 ± 2.3
Benzyl viologen without an electron donor < 0.20
Formate and benzyl viologen 1.24 ± 1.0
Formate and PMS/DCPIP 638.3 ± 69
  1. a The buffer used was 50 mM sodium phosphate pH 7.2; BV was used at a final concentration of 4 mM; formate was added to a final concentration of 18 mM; and PMS/DCPIP were added at final concentrations of 20 μM and 78 μM, respectively.
  2. b The mean and standard deviation (±) of at least three independent experiments are shown.