Figure 7

Possible interaction of HSP90 and SSCMK1. Evidence from RNAi inhibition of SSCMK1, HSP90 inhibition with GdA and yeast two-hybrid assay presented in this work suggests that SSCMK1 could affect fungal thermotolerance by its interaction with SSHSP90. SSCMK1 was found to interact with the C terminal domain of SSHSP90, where effectors of this heat shock protein interact. HSP90 has been identified as interacting with phosphatase, calcineurin and other kinases in many other fungal systems. The interaction of HSP90 with these proteins involves the N terminal domain. The interaction of HSP90 with calcineurin would in turn modulate the activity of SSCMK1. The presence and interaction of calcineurin in S. schenckii is at the moment expeculative because this protein has not been described in this fungus.