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Table 1 Oligonucleotide primers used to assemble short DNA fragments coding for wild-type and truncated epitope sequences

From: Identification of two linear B-cell epitopes from West Nile virus NS1 by screening a phage-displayed random peptide library

Designations of primers

Sequences of primers

Sequences of coded peptides (designations)

Cp-1-F

5'-AATTCctcaccgccaccacggaaaaaTAAG-3'

LTATTEK (Cp-1)

Cp-1-R

5'-TCGACTTAtttttccgtggtggcggtgagG-3'

 

Cp-2-F

5'-AATTCaccgccaccacggaaaaaTAAG-3'

TATTEK (Cp-2)

Cp-2-R

5'-TCGACTTAtttttccgtggtggcggtG-3'

 

Cp-3-F

5'-AATTCctcaccgccaccacggaaTAAG-3'

LTATTE (Cp-3)

Cp-3-R

5'-TCGACTTAttccgtggtggcggtgagG-3'

 

Cp-4-F

5'-AATTCgccaccacggaaaaaTAAG-3'

ATTEK (Cp-4)

Cp-4-R

5'-TCGACTTAtttttccgtggtggcG-3'

 

Cp-5-F

5'-AATTCctcaccgccaccacgTAAG-3'

LTATT (Cp-5)

Cp-5-R

5'-TCGACTTAcgtggtggcggtgagG-3'

 

Dp-1-F

5'-AATTCgtggttgatggtccggagaccaaggaatgtTAAG-3'

VVDGPETKEC (Dp-1)

Dp-1-R

5'-TCGACTTAacattccttggtctccggaccatcaaccacG-3'

 

Dp-2-F

5'-AATTCgttgatggtccggagaccaaggaatgtTAAG-3'

VDGPETKEC (Dp-2)

Dp-2-R

5'-TCGACTTAacattccttggtctccggaccatcaacG-3'

 

Dp-3-F

5'-AATTCgtggttgatggtccggagaccaaggaaTAAG-3'

VVDGPETKE (Dp-3)

Dp-3-R

5'-TCGACTTAttccttggtctccggaccatcaaccacG-3'

 

Dp-4-F

5'-AATTCgatggtccggagaccaaggaatgtTAAG-3'

DGPETKEC (Dp-4)

Dp-4-R

5'-TCGACTTAacattccttggtctccggaccatcG-3'

 

Dp-5-F

5'-AATTCgtggttgatggtccggagaccaagTAAG-3'

VVDGPETK (Dp-5)

Dp-5-R

5'-TCGACTTActtggtctccggaccatcaaccacG-3'

 

Dp-6-F

5'-AATTCggtccggagaccaaggaatgtTAAG-3'

GPETKEC (Dp-6)

Dp-6-R

5'-TCGACTTAacattccttggtctccggaccG-3'

 

Dp-7-F

5'-AATTCgtggttgatggtccggagaccTAAG-3'

VVDGPET (Dp-7)

Dp-7-R

5'-TCGACTTAggtctccggaccatcaaccacG-3'

 
  1. Notes: Nucleotides introduced to form the overhanging ends of EcoR I and Sal I, and the TAA stop codon are shown in italic letters.