|
Antibiotic
|
MICa
|
Fold MIC decrease in BB255ΔVraRb
|
Lag before inductionc
|
Maximum inductiond
|
Time point of maximum inductione
|
Concentration dependencef
|
OD/CFU/ml as % of controlg
|
|---|
|
Fosfomycin
|
0.5
|
2x
|
30
|
high
|
120
|
high (29.5)
|
47/10
|
|
D-Cycloserine
|
12
|
none
|
10
|
medium
|
60
|
high (25.5)
|
56/36
|
|
Bacitracin
|
32
|
10x
|
none
|
medium
|
60
|
low (1.5)
|
26/9
|
|
Tunicamycin
|
8
|
4x
|
10
|
high
|
120
|
medium (3.0)
|
38/9
|
|
Flavomycin
|
4
|
16x
|
none
|
high
|
60
|
low (1.6)
|
41/25
|
|
Vancomycin
|
1.3
|
2x
|
none
|
low
|
120
|
medium (12.6)
|
100/100
|
|
Oxacillin
|
0.2
|
none
|
none
|
high
|
120
|
high (19.1)
|
74/20
|
|
Daptomycin
|
0.25
|
2x
|
none
|
low
|
120
|
medium (14.1)
|
85/75
|
|
Lysostaphin
|
0.065
|
2x
|
none
|
low
|
10
|
medium (11.3)
|
11/6
|
|
Teicoplanin
|
0.5
|
10x
|
none
|
medium
|
60
|
medium (7.5)
|
91/83
|
- aDetermined in μg/ml for BB255 psas016-luc+. bDifference in MIC values of BB255/BB255ΔVraR. cEarliest time point at which induction was detected (min). dInduction levels were scored as: high (> 40'000 RLU); medium (>10'000 - < 40'000); low (< 10'000). eTime taken for maximum induction to be reached after antibiotic addition (min). fThe ratio of maximal induction levels measured at 5x MIC/0.2x MIC, scored as: high (> 15); medium (>2 - < 15); low (< 2). gOD and CFU/ml values after treatment with antibiotics (1x MIC) for 120 min, expressed as a percentage of the values from untreated cell.
