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Table 1 Proteins identified by HPLC-MS/MS

From: Staphylococcus aureus Biofilm and Planktonic cultures differentially impact gene expression, mapk phosphorylation, and cytokine production in human keratinocytes

Band # Sample NCBI Accession Name Function
1 BCM gi15924466 30S ribosomal protein S1 [Staphylococcus aureus subsp. aureus Mu50] translation
1 BCM gi227557405 elongation factor G [Staphylococcus aureus subsp. aureus MN8] translation
2 BCM gi15923949 glycerophosphoryl diester hosphodiesterase [Staphylococcus aureus subsp. aureus Mu50] glycerophospholipid metabolism
3 BCM gi15924653 valyl-tRNA synthetase [Staphylococcus aureus subsp. aureus Mu50] translation
4 BCM gi258423763 isoleucyl-tRNA synthetase Staphylococcus aureus A9635] translation
5 BCM gi2506027 N-acetyl-glucosaminidase [Staphylococcus aureus] exoglycosidase
6 BCM gi15924060 amidophosphoribosyltransferase Staphylococcus aureus subsp. aureus Mu50] purine nucleotide biosynthesis
7 BCM gi128852 Staphylococcal nuclease nuclease
8 BCM No significant hits NA NA
9 BCM gi258424814 catalase [Staphylococcus aureus A9635] antioxidant/oxidative stress
9 BCM gi21282950 catalase [Staphylococcus aureus subsp. aureus MW2] antioxidant/oxidative stress
10 BCM No significant hits NA NA
11 BCM No significant hits NA NA
12 BCM&PCM gi15925406 phosphoglycerate mutase [Staphylococcus aureus subsp. aureus Mu50] glycolysis
12 BCM&PCM gi282917765 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase [Staphylococcus aureus subsp. aureus D139] glycolysis
12 BCM&PCM gi|15927092 6-phosphogluconate dehydrogenase [Staphylococcus aureus subsp. aureus N315] Pentose phosphate
    bifunctional 3-deoxy-7-hosphoheptulonate  
12 BCM&PCM gi15924727 synthase/chorismate mutase [Staphylococcus aureus subsp. shikimate pathway
    aureus Mu50]  
12 BCM&PCM gi15923310 glycerol ester hydrolase [Staphylococcus aureus subsp. aureus Mu50] lipase
13 BCM&PCM gi15924543 superoxide dismutase [Staphylococcus aureus subsp. aureus Mu50] antioxidant/oxidative stress
14 BCM&PCM gi15923346 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase [Staphylococcus aureus subsp. aureus Mu50] methionine metabolism
14 BCM&PCM gi293501167 aconitate hydratase 1 [Staphylococcus aureus subsp. aureus 58-424] TCA
15 PCM gi15925596 fructose-1,6-bisphosphate aldolase [Staphylococcus aureus subsp. aureus Mu50] glycolysis
16 PCM gi15923621 lipoprotein [Staphylococcus aureus subsp. aureus Mu50] cell wall component
16 PCM gi15925115 fructose-bisphosphate aldolase [Staphylococcus aureus subsp. aureus Mu50] glycolysis
17 PCM gi289550260 fructose-bisphosphate aldolase class II [Staphylococcus lugdunensis HKU09-01] glycolysis
17 PCM gi283470068 phosphoglycerate kinase [Staphylococcus aureus subsp. aureus ST398] glycolysis
18 PCM gi15923952 glucose-6-phosphate isomerase [Staphylococcus aureus subsp. aureus Mu50] glycolysis
18 PCM gi15923762 glyceraldehyde-3-phosphate dehydrogenase [Staphylococcus aureus subsp. aureus Mu50] glycolysis
18 PCM gi151221290 ornithine carbamoyltransferase [Staphylococcus aureus subsp. aureus str. Newman] urea cycle
  1. Proteins identified by HPLC-MS/MS analysis. Band numbers represent excised bands from 1D-SDS PAGE analysis of BCM and PCM (Figure 1).