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Table 1 Examples of Ftp library clones that express adhesive polypeptides

From: Adhesive polypeptides of Staphylococcus aureus identified using a novel secretion library technique in Escherichia coli

Clone Name Length of insert* Chromosomal location of insert† ORFs‡ in insert Predicted gene product(s) of the Ftp-clone Presence of FliC1-20 and/or FLAG-tag in the gene product Binding specificity of the product Predicted molecular mass#
ΔNarG 393 2465481-2465873 1) 02681 NarG §1 FliC 1-20
FLAG-tag 		None 18.5
ΔFnBPA 346 2581863-2582208 1) 02803 FnBPA §2 FliC 1-20
FLAG-tag 		Fn 16.6
ΔEbh 582 1398633-1399214 1) 01447 Ebh §2 FliC 1-20
FLAG-tag 		Fn 24.2
ΔCoa 825 212434-213258 1) 00192 coagulase FliC 1-20
FLAG-tag 		Fg, Fn 34.2
ΔPurK 383 979768-980150 1) 01008 out of frame No   
    2) 01009 PurK §1 FLAG-tag Fn, Fg 14.6
ΔSCOR 484 2667518-2668001 1) 02897 terminator in sequence FliC   
    2) 02898 Putative SCOR §1 FLAG-tag Fn, Fg 17.7
ΔUsp 664 1724620-1725283 1) 01818 out of frame No   
    2) 01819 Usp §1 -like FLAG-tag Fn, Fg, CIV, 19.3
ΔIspD 885 244692-245576 1) 00223 out of frame No   
    2) 00225 IspD §2 FLAG-tag Fn, Fg 13.4
ΔPBP 756 2257336-2258091 1) 02433 out of frame No   
    2) 02432 out of frame No   
    3) 02430 putative PBP §1
of ABC §1 transporter 		FLAG-tag Fn, Fg 6.7
  1. * In base pairs
  2. † In S. aureus subsp. aureus NCTC 8325
  3. ‡ Open reading frames (ORFs) in the clones are partial, the number refers to the systematic gene identifier SAOUHSC_no. in the GenBank database, a locus_tag
  4. §1 Abbreviations of TIGR Family names: NarG, nitrate reductase α-subunit; PurK, Phosphoribosylamino-imidazole carboxylase ATPase subunit; SCOR, short-chain oxidoreductase; Usp, universal stress protein family; PBP, periplasmic binding protein; ABC, ATP-binding cassette
  5. §2 Abbreviations of the protein names: FnBPA, fibronectin binding protein A; Ebh, extracellular matrix binding protein homologue; IspD, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
  6. The reading frame is in relation to fliC and flag sequences
  7. # Molecular mass in kilodaltons. The molecular mass of FliC1-20 and FLAG-tag included when present in the gene product
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BMC Microbiology

ISSN: 1471-2180

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