Lack of evidence for a role of hydrophobins in conferring surface hydrophobicity to conidia and hyphae of Botrytis cinerea

Table 1 Sequence characteristics of B. cinerea hydrophobins and hydrophobin-like proteins.

Name/predicted class	Size	Spacing of cysteine residues						GRAVY
Bhp1 (BC1G_15273)	111/93	N- 34-C- 7	-CC- 18	-C- 15	-C- 5	-CC- 17	-C- 7	0.57
Consensus spacing class I		N- Xn-C- (5-8)	-CC-(17-39)	-C-(8-23)	-C-(5-6)	-CC-(6-18)	-C-(2-13)
Bhp2 (BC1G_03994)	98/77	N- 33-C- 6	-CC- 11	-C- 16	-C- 8	-CC- 10	-C- 6	0.42
Bhp3 (BC1G_01012)	98/80	N- 34-C- 8	-CC- 11	-C- 16	-C- 8	-CC- 10	-C- 3	0.30
Consensus spacing class II		N- Xn-C-(9-10)	-CC- 11	-C- 16	-C-(6-9)	-CC- 10	-C- (3-7)
Bhl1 (BC1G_01003)	145/125	N- 60-C- 9	-CC- 31	-C- 8	-C- 7	-CC- 16	-C- 6	0.76
BC1G_02483	234/211	N- 82-C- 8	-CC- 7	-C- 5	-C- 9	-CC- 8	-C- 107	-0.10
BC1G_03277	178/160	N-111-C- 7	-CC- 10	-C- 17	-C- 8	-CC- 12	-C- 5	-0.43
BC1G_04521	181/157	N-120-C- 7	-CC- 10	-C- 10	-C- 9	-CC- 4	-C- 13	0.01
BC1G_11117	109/88	N- 35-C- 10	-CC- 15	-C- 18	-C- 8	-CC- 11	-C- 4	-0.77
BC1G_12747	106/86	N- 37-C- 3	-CC- 10	-C- 13	-C- 18	-CC- 4	-C- 13	-0.28

For the three hydrophobins Bhp1 (class I), Bhp2 and Bhp3 (both class II), and for six hydrophobin-like proteins, the cysteine spacing is shown. Consensus cysteine spacings for class I and class II proteins were taken from [16]. The sizes (amino acids) of the unprocessed and processed proteins are indicated. N: N-terminus; Xn: Undefined number of amino acids; Underlined: Strictly conserved spacing; GRAVY: Grand average of hydropathicity of the region covering the eight cysteines. Positive GRAVY values indicate hydrophobicity [53].

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