Table 1 MLST and mouse assay of L. innocua and L. monocytogenes strains
Strain | Source | Subgroup/ lineage (serovar) | rhamnose | Allele designation | Virulence gene | Mouse assay | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
gyrB | dapE | hisJ | sigB | ribC | purM | betL | gap | tuf | STa | bsh | inlJ | CFU | Relative virulence (%) | ||||||
L. innocua | |||||||||||||||||||
ATCC33090 | reference | A | + | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | -- | -- | 3.5 × 107 | 0 | ||
90001 | reference | B | + | 2 | 2 | 2 | 2 | 2 | 2 | 1 | 1 | 2 | 2 | -- | -- | 2.7 × 107 | 0 | ||
1603 | reference | B | + | 3 | 3 | 3 | 3 | 3 | 3 | 2 | 1 | 2 | 3 | + | -- | 2.0 × 107 | 0 | ||
AB2497 | reference | A | + | 1 | 1 | 4 | 1 | 1 | 1 | 1 | 1 | 1 | 4 | -- | -- | 3.3 × 107 | 0 | ||
CLIP11262 | reference | A | + | 1 | 4 | 5 | 1 | 1 | 4 | 3 | 1 | 1 | 5 | -- | -- | ND | ND | ||
0063 | meat | C | + | 4 | 5 | 6 | 4 | 4 | 5 | 4 | 1 | 1 | 6 | -- | -- | 5.3 × 107 | 0 | ||
0065 | meat | A | + | 1 | 6 | 5 | 1 | 1 | 4 | 3 | 1 | 1 | 7 | -- | -- | 1.5 × 107 | 0 | ||
0068 | meat | B | + | 2 | 2 | 5 | 2 | 5 | 2 | 5 | 1 | 2 | 8 | -- | -- | 1.8 × 107 | 0 | ||
0072 | meat | A | + | 1 | 7 | 5 | 5 | 1 | 4 | 3 | 2 | 1 | 9 | -- | -- | 2.1 × 107 | 0 | ||
0082 | meat | A | + | 1 | 7 | 5 | 1 | 1 | 4 | 3 | 1 | 1 | 10 | -- | -- | 2.3 × 107 | 0 | ||
0083 | meat | A | + | 1 | 8 | 5 | 1 | 1 | 4 | 3 | 1 | 1 | 11 | -- | -- | 1.7 × 107 | 0 | ||
0173 | meat | A | + | 4 | 9 | 1 | 6 | 6 | 1 | 6 | 1 | 1 | 12 | -- | -- | 2.2 × 107 | 0 | ||
0197 | meat | A | + | 4 | 10 | 1 | 6 | 6 | 1 | 6 | 1 | 1 | 13 | -- | -- | 1.9 × 107 | 0 | ||
01174 | meat | A | + | 5 | 11 | 7 | 7 | 7 | 4 | 1 | 1 | 1 | 14 | -- | -- | 1.3 × 107 | 0 | ||
01178 | meat | A | + | 1 | 12 | 5 | 1 | 1 | 4 | 3 | 1 | 1 | 15 | -- | -- | 3.3 × 107 | 0 | ||
01182 | meat | A | + | 1 | 13 | 5 | 1 | 1 | 4 | 3 | 1 | 1 | 16 | -- | -- | 2.3 × 107 | 0 | ||
317 | milk | A | + | 1 | 1 | 5 | 1 | 1 | 1 | 7 | 1 | 1 | 17 | -- | -- | 3.3 × 107 | 0 | ||
337 | milk | B | + | 3 | 3 | 3 | 3 | 3 | 3 | 2 | 1 | 2 | 3 | -- | -- | 2.0 × 107 | 0 | ||
376 | milk | A | + | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | -- | -- | 2.5 × 107 | 0 | ||
380 | milk | B | + | 3 | 3 | 3 | 3 | 8 | 3 | 2 | 1 | 2 | 18 | -- | -- | 2.1 × 107 | 0 | ||
386 | milk | B | -- | 5 | 14 | 8 | 8 | 9 | 4 | 8 | 1 | 1 | 19 | + | -- | 3.0 × 107 | 0 | ||
438 | milk | B | + | 6 | 15 | 9 | 9 | 10 | 6 | 3 | 3 | 1 | 20 | -- | -- | 1.6 × 107 | 0 | ||
693 | milk | A | + | 1 | 12 | 7 | 1 | 1 | 4 | 3 | 1 | 1 | 21 | -- | -- | 2.3 × 107 | 0 | ||
694 | milk | A | + | 5 | 11 | 1 | 7 | 11 | 4 | 9 | 1 | 1 | 22 | -- | -- | 4.7 × 107 | 0 | ||
ZS14 | seafood | A | + | 1 | 12 | 5 | 1 | 1 | 7 | 10 | 1 | 1 | 23 | -- | -- | 4.3 × 107 | 0 | ||
ZXF | seafood | B | + | 3 | 3 | 3 | 3 | 12 | 3 | 2 | 1 | 2 | 24 | -- | -- | 3.3 × 107 | 0 | ||
1571 | seafood | A | + | 1 | 16 | 5 | 1 | 1 | 4 | 3 | 1 | 1 | 25 | -- | -- | 2.0 × 107 | 0 | ||
L19 | seafood | B | -- | 7 | 17 | 10 | 10 | 13 | 8 | 11 | 4 | 2 | 26 | -- | -- | 1.5 × 107 | 0 | ||
L43 | seafood | D | + | 8 | 18 | 11 | 11 | 14 | 9 | 12 | 5 | 3 | 27 | -- | + | 1.7 × 107 | 0 | ||
NB2 | seafood | B | + | 3 | 3 | 3 | 3 | 15 | 3 | 2 | 1 | 2 | 28 | -- | -- | 3.5 × 107 | 0 | ||
NB3 | seafood | B | + | 3 | 3 | 3 | 3 | 15 | 3 | 2 | 1 | 2 | 28 | -- | -- | 3.7 × 107 | 0 | ||
NB24 | seafood | B | + | 5 | 19 | 12 | 12 | 16 | 2 | 13 | 1 | 1 | 29 | -- | -- | 2.9 × 107 | 0 | ||
L87 | pork | B | + | 5 | 19 | 12 | 7 | 16 | 10 | 13 | 1 | 1 | 30 | -- | -- | 1.3 × 107 | 0 | ||
L103 | chicken | A | -- | 1 | 12 | 5 | 1 | 1 | 11 | 14 | 1 | 1 | 31 | -- | -- | 4.0 × 107 | 0 | ||
L. monocytogenes | |||||||||||||||||||
SH3 | pork | I (1/2b) | + | 9 | 20 | 13 | 13 | 17 | 12 | 15 | 6 | 4 | 32 | + | + | 4.3 × 107 | 100 | ||
NB26 | seafood | I (1/2b) | + | 10 | 21 | 14 | 14 | 18 | 13 | 16 | 6 | 4 | 33 | + | + | 6.5 × 107 | 100 | ||
NB27 | seafood | I (1/2b) | + | 11 | 22 | 15 | 14 | 19 | 14 | 17 | 7 | 4 | 34 | + | + | 5.5 × 107 | 80 | ||
M1 | milk | I (1/2b) | + | 11 | 23 | 13 | 14 | 19 | 15 | 18 | 8 | 4 | 35 | + | + | 3.0 × 107 | 100 | ||
ScottA | reference | I (4b) | + | 11 | 24 | 15 | 15 | 19 | 16 | 19 | 8 | 4 | 36 | + | + | 3.3 × 107 | 100 | ||
NB4 | seafood | I (4b) | + | 11 | 25 | 16 | 15 | 19 | 14 | 20 | 6 | 4 | 37 | + | + | 2.1 × 107 | 100 | ||
NB6 | seafood | I (4b) | + | 11 | 26 | 17 | 16 | 20 | 16 | 21 | 6 | 4 | 38 | + | + | 3.3 × 107 | 100 | ||
NB7 | seafood | I (4b) | + | 11 | 26 | 17 | 16 | 20 | 16 | 22 | 6 | 4 | 39 | + | + | 4.6 × 107 | 100 | ||
NB25 | seafood | I (4b) | + | 11 | 27 | 18 | 15 | 19 | 17 | 19 | 6 | 4 | 40 | + | + | 4.6 × 107 | 100 | ||
90SB1 | animal | I (4b) | + | 11 | 27 | 16 | 15 | 19 | 17 | 19 | 6 | 4 | 41 | + | + | 5.5 × 107 | 100 | ||
EGDe | reference | II (1/2a) | + | 12 | 28 | 19 | 17 | 21 | 18 | 20 | 9 | 5 | 42 | + | + | 5.5 × 107 | 100 | ||
10403S | reference | II (1/2a) | + | 13 | 29 | 20 | 17 | 22 | 19 | 20 | 10 | 5 | 43 | + | + | 5.0 × 107 | 100 | ||
SH2 | vegetable | II (1/2a) | + | 13 | 29 | 21 | 17 | 23 | 20 | 20 | 9 | 5 | 44 | + | + | 4.3 × 107 | 100 | ||
SH4 | chicken | II (1/2a) | + | 13 | 29 | 20 | 17 | 22 | 19 | 20 | 11 | 5 | 45 | + | + | 5.0 × 107 | 100 | ||
NB5 | seafood | II (1/2a) | + | 13 | 29 | 22 | 17 | 24 | 21 | 23 | 12 | 5 | 46 | + | + | 4.5 × 107 | 100 | ||
NB21 | seafood | II (1/2a) | + | 13 | 29 | 23 | 17 | 25 | 22 | 23 | 13 | 5 | 47 | + | + | 3.9 × 107 | 80 | ||
P3 | pork | II (1/2a) | + | 13 | 23 | 24 | 18 | 26 | 23 | 24 | 13 | 5 | 48 | + | + | 4.0 × 107 | 100 | ||
NB28 | seafood | II (1/2c) | + | 12 | 23 | 19 | 17 | 21 | 18 | 20 | 14 | 6 | 49 | + | + | 4.1 × 107 | 100 | ||
V1 | vegetable | II (1/2c) | + | 12 | 23 | 19 | 17 | 21 | 18 | 20 | 9 | 6 | 50 | + | + | 3.0 × 107 | 100 | ||
P19 | chicken | II (1/2c) | + | 12 | 30 | 19 | 17 | 21 | 18 | 20 | 9 | 6 | 51 | + | + | 5.0 × 107 | 100 | ||
54006 | reference | IIIA (4a) | + | 14 | 31 | 25 | 19 | 27 | 24 | 3 | 15 | 2 | 52 | + | -- | 1.3 × 107 | 0 | ||
F2-695 | reference | IIIA(4a) | + | 15 | 32 | 26 | 20 | 28 | 25 | 25 | 15 | 7 | 53 | + | + | 1.2 × 107 | 40 | ||
F2-086 | reference | IIIB (4a) | -- | 16 | 33 | 27 | 21 | 29 | 26 | 26 | 16 | 8 | 54 | + | -- | 1.7 × 107 | 100 | ||
F2-407 | reference | IIIB (4a) | -- | 17 | 34 | 28 | 21 | 30 | 26 | 27 | 16 | 9 | 55 | + | -- | 1.5 × 107 | 100 | ||
F2-270 | reference | IIIB (4a) | -- | 18 | 35 | 29 | 21 | 31 | 27 | 28 | 16 | 8 | 56 | + | -- | 2.2 × 107 | 100 | ||
F2-208 | reference | IIIC (4a) | -- | 19 | 36 | 30 | 22 | 32 | 28 | 29 | 16 | 10 | 57 | + | -- | 3.5 × 107 | 100 | ||
F2-525 | reference | IIIA (4b) | + | 20 | 37 | 31 | 23 | 33 | 29 | 30 | 17 | 11 | 58 | + | + | 2.8 × 107 | 100 | ||
J1-158 | reference | IIIB (4b) | -- | 21 | 34 | 28 | 21 | 29 | 30 | 31 | 16 | 8 | 59 | + | -- | 2.2 × 107 | 40 | ||
J2-071 | reference | IIIA (4c) | + | 22 | 38 | 26 | 20 | 28 | 31 | 25 | 15 | 12 | 60 | + | + | 1.5 × 107 | 100 | ||
W1-111 | reference | IIIC (4c) | -- | 23 | 39 | 32 | 24 | 34 | 32 | 32 | 18 | 2 | 61 | + | -- | 2.8 × 107 | 80 | ||
L. welshimeri | |||||||||||||||||||
SLCC5334 | reference | ND | -- | 24 | 40 | 33 | 25 | 35 | 33 | 33 | 19 | 13 | 62 | -- | -- | ND | ND | ||
C15 | reference | ND | -- | 25 | 41 | 34 | 26 | 36 | 34 | 34 | 20 | 14 | 63 | -- | -- | 5.5 × 107 | 0 | ||
AB1626 | reference | ND | -- | 25 | 41 | 35 | 27 | 36 | 34 | 34 | 20 | 14 | 64 | -- | -- | ND | ND | ||
AB2500 | reference | ND | -- | 24 | 40 | 36 | 25 | 35 | 33 | 33 | 19 | 13 | 65 | -- | -- | ND | ND | ||