Table 3 Ability of H. pylori SmpB and of wild type or mutant alleles of ssrA^Hpto support growth of λimm^P22 in E. coli ΔssrA or ΔsmpB deletion mutants and to restore the growth defect in E. coli ΔssrA or ΔsmpB mutants

MG1655

smpB ^Ec ssrA ^Ec

1

-

MG1655 ΔsmpB pILL2150

ΔsmpB^Ec ssrA^Ec

1.3 × 10^-5

no

MG1655 ΔsmpB pILL786

ΔsmpB^Ec ssrA^Ec/smpB^Hp

0.6

yes

MG1655 ΔssrA pILL2150

smpB^EcΔssrA^Ec

2.6 × 10^-5

no

MG1655 ΔssrA pILL2334

smpB^Ec ΔssrA^Ec/ssrA^Ec-WT

1

yes

MG1655 ΔssrA pILL788

smpB^Ec ΔssrA^Ec/ssrA^Hp-WT

5.0 × 10^-5

yes

MG1655 ΔssrA pILL791

smpB^Ec ΔssrA^Ec/ssrA^Hp-DD

1.6 × 10^-5

yes

MG1655 ΔssrA pILL2328

smpB^Ec ΔssrA^Ec/ssrA^Hp-STOP

6.1 × 10^-5

no

MG1655 ΔssrA pILL792

smpB^Ec ΔssrA^Ec/ssrA^Hp-resume

3.9 × 10^-5

no

MG1655 ΔssrA pILL793

smpB^Ec ΔssrA^Ec/ssrA^Hp-wobble

2.3 × 10^-5

no

MG1655 ΔssrA pILL794

smpB^Ec ΔssrA^Ec/ssrA^Hp-smpB

3.6 × 10^-5

No