Figure 1
From: PCR-based method for targeting 16S-23S rRNA intergenic spacer regions among Vibrio species
This figure shows the successful elimination of heteroduplex artifacts following secondary PCR process. Lanes one, three and five show IGS-pattern results following the initial PCR. Lanes two, four and six show IGS-type patterns for the same samples after completion of the one extra PCR amplification step. Lanes 1-2, V. cholerae ATCC 25874; lanes 3-4, V. vulnificus ATCC 43382; Lanes 5-6, V. parahaemolyticus ATCC 17802. Lane L, MW ladder.