Interactions between endocarditis-derived Streptococcus gallolyticus subsp. gallolyticus isolates and human endothelial cells

Table 2 Primer sequences and PCR conditions.

Primer	Oligonucleotide sequence (5'-3')	Nucleotide positions*	Annealing temperature	Amplicon length	Genbank accession no.
fimB-550F	GGTAAGTGATGGTATTGATGTC	550-571	45	347	AY321316
fimB-875R	GTGTTCCTTCTTCCTCAGTATT	875-896
gtf-F	GGTGAGACTTGGGTTGATTC	2049-2068	54	496	AB292595
gtf-R	GCTCTGCTTGAACAACTGGA	2525-2544
pilB-385F	AAGGGACGAGGGCTCTAC	120017-120034	58	339	CP000408
pilB-722R	ACCCAATTCCAACATACG	120373-120356

ISSN: 1471-2180