Figure 1

FT binds to PLG from human plasma. FTLVS cultured to mid-log phase in BHI broth were bound to wells of microtiter plates and then incubated for 1 hour with fresh frozen human plasma (FFP) in the presence or absence of 100 mM ε-amino caproic acid (εACA), a PLG-binding inhibitor. A modified ELISA was performed to measure FTLVS-bound PLG. The results shown are representative of 3 experiments of similar design. Bars indicate +/- SEM in triplicate. Statistical analysis was performed via one-way ANOVA using a Dunnett's Multiple Comparison post-test (*** P < .001).