Figure 6
From: Modulation of iron homeostasis in macrophages by bacterial intracellular pathogens
Expression of genes involved in iron homeostasis during infection with Francisella or Salmonella. RAW264.7 macrophages were infected for 24 h with wild-type Francisella (A), wild type Salmonella (B), spiC Salmonella (C), or spiA Salmonella (D). Quantitative mRNA levels were determined by quantitative light cycler PCR for: iron-regulatory protein 1 (IRP1), iron regulatory protein 2 (IRP2), ferrireductase (Steap3), transmembrane iron transporter (Dmt1), lipocalin (Lcn2), lipocalin receptor (LcnR), ferroportin (Fpn1), antimicrobial peptide hepcidin (Hamp1), heme oxygenase (Hmox1), ferritin heavy chain 1(Fth1), ferritin light chain 1 (Ftl1), and ferritin light chain 2 (Ftl2). Measurements were standardized to GAPDH-mRNA levels for each experiment. Values shown represent the ratio of mRNA for a given gene in infected cells divided by the mRNA level in uninfected cells (mRNA infected/mRNA uninfected). Statistically significant expression data are shown by solid bars (Student's t-test, p < 0.05 is considered as significant; individual p-values are given in the text). Results from n = 6 experiments are expressed as means +/- 1 standard error of mean (SEM).