Location of the mRNA 5'ends for the hdrE1, hdrA1 , mrpA, fpoP, pta, aceP , and ahaA genes. Top panel; Sequence gels for the mrpA, fpoP, ahaA and aceP genes along with the corresponding DNA ladders. RNA prepared from methanol or from acetate-grown cells is indicated by Me and Ac, respectively. Bottom panel: the alignment of the upstream DNA sequences relative to the start of transcription (+1 position). The position of the initiation codon is boxed where the numbering is relative to the start of transcription. The putative TATA-box sequences are double underlined and the BRE-regions are indicated by a solid underline. The mRNA 5' end positions for the pta, hdrA1, and hdrE1 genes were determined with a ubiquitous ladder (data not shown).