Virulence of P. luminescens to insect larvae. TT01gfp and mutant strains were grown overnight in LB broth at 30°C and diluted in PBS so that approximately 200 cfu were injected into each of 10 final-instar G. mellonella larvae. The insects were incubated at 25°C and insect death was monitored over the next 72 hours. In each graph the virulence of the mutant (■) is compared to TT01gfp (□). The results shown are of a representative experiment that was independently repeated at least 3 times.