Interaction of different CspA orthologs to interact with animal CFH. Purified recombinant CspA and B. garinii ST4 CspA orthologs were subjected to 10% Tris/Tricine SDS-PAGE and blotted to nitrocellulose membranes. Recombinant proteins were visualized by an anti-GST antibody. Additional membranes were incubated with sera obtained from diverse animals. Interacting proteins were then visualized using a polyclonal anti-CFH antibody.