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Figure 8 | BMC Microbiology

Figure 8

From: Identification and functional characterisation of Complement Regulator Acquiring Surface Protein-1 of serum resistant Borrelia garinii OspA serotype 4

Figure 8

Interaction of different CspA orthologs to interact with animal CFH. Purified recombinant CspA and B. garinii ST4 CspA orthologs were subjected to 10% Tris/Tricine SDS-PAGE and blotted to nitrocellulose membranes. Recombinant proteins were visualized by an anti-GST antibody. Additional membranes were incubated with sera obtained from diverse animals. Interacting proteins were then visualized using a polyclonal anti-CFH antibody.

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