Table 2 Real-time PCR quantification of bifidobacteria and lactobacilli
From: Impact of a synbiotic food on the gut microbial ecology and metabolic profiles
Subject | Time point | 16S rrn operons/μg fecal genomic DNA (mean ± SD) | |||
|---|---|---|---|---|---|
| Â | Â | Bifidobacterium | B. longum | Lactobacillus | L. helveticus |
1 | T0 | 9.4 × 106 ± 3.7 × 106 | 3.2 × 106 ± 1.5 × 106 | 2.6 × 106 ± 9.6 × 105 | 0.0 ± 0.0 |
|  | T1 | 4.1 × 106 ± 8.3 × 105 | 1.1 × 106 ± 2.9 × 105 | 1.9 × 106 ± 9.9 × 105 | 4.5 × 102 ± 2.9 × 102 |
2 | T0 | 8.9 × 107 ± 3.1 × 107 | 4.2 × 107 ± 3.6 × 107 | 1.1 × 105 ± 5.6 × 104 | 9.0 × 101 ± 6.2 × 101 |
|  | T1 | 1.6 × 107 ± 5.0 × 106 | 4.7 × 106 ± 2.9 × 105 | 5.1 × 105 ± 2.4 × 105 | 2.6 × 103 ± 2.8 × 102 |
3 | T0 | 4.0 × 108 ± 3.6 × 107 | 8.6 × 106 ± 2.6 × 106 | 5.6 × 104 ± 3.5 × 104 | 0.0 ± 0.0 |
|  | T1 | 2.4 × 108 ± 2.5 × 107 | 2.4 × 107 ± 2.9 × 106 | 2.6 × 105 ± 1.6 × 105 | 2.8 × 103 ± 1.8 × 103 |
4 | T0 | 2.6 × 108 ± 2.8 × 107 | 2.3 × 107 ± 2.9 × 106 | 1.6 × 105 ± 1.0 × 103 | 2.1 × 103 ± 8.7 × 101 |
|  | T1 | 5.8 × 108 ± 1.2 × 107 | 3.7 × 107 ± 3.1 × 106 | 1.2 × 105 ± 2.7 × 104 | 1.6 × 103 ± 2.2 × 102 |
5 | T0 | 3.1 × 106 ± 8.6 × 105 | 9.8 × 105 ± 2.8 × 105 | 1.9 × 104 ± 5.8 × 103 | 0.0 ± 0.0 |
|  | T1 | 2.4 × 106 ± 7.3 × 105 | 9.5 × 105 ± 3.4 × 105 | 6.1 × 104 ± 3.4 × 104 | 3.5 × 102 ± 2.3 × 102 |
6 | T0 | 1.7 × 108 ± 3.8 × 107 | 6.5 × 106 ± 2.4 × 105 | 2.7 × 105 ± 1.2 × 105 | 0.0 ± 0.0 |
|  | T1 | 6.2 × 108 ± 4.2 × 107 | 3.5 × 107 ± 2.0 × 105 | 1.7 × 105 ± 1.1 × 105 | 0.0 ± 0.0 |
7 | T0 | 6.4 × 107 ± 4.8 × 106 | 3.4 × 107 ± 1.2 × 106 | 4.0 × 105 ± 1.7 × 105 | 9.0 × 101 ± 8.2 × 101 |
|  | T1 | 7.5 × 107 ± 1.2 × 106 | 4.6 × 107 ± 5.5 × 106 | 9.2 × 105 ± 4.9 × 105 | 1.4 × 104 ± 3.2 × 103 |
8 | T0 | 1.8 × 106 ± 5.8 × 105 | 6.0 × 105 ± 3.6 × 105 | 1.0 × 106 ± 1.0 × 106 | 0.0 ± 0.0 |
|  | T1 | 4.1 × 106 ± 8.5 × 105 | 1.3 × 106 ± 9.7 × 105 | 1.7 × 105 ± 1.7 × 105 | 0.0 ± 0.0 |
9 | T0 | 4.4 × 106 ± 2.8 × 105 | 3.0 × 106 ± 2.3 × 106 | 9.2 × 105 ± 9.0 × 105 | 3.0 × 103 ± 1.1 × 103 |
|  | T1 | 5.6 × 106 ± 1.4 × 105 | 3.8 × 106 ± 1.3 × 106 | 2.0 × 106 ± 1.0 × 106 | 1.8 × 103 ± 1.7 × 103 |
10 | T0 | 1.0 × 108 ± 1.8 × 107 | 7.0 × 107 ± 4.5 × 107 | 7.7 × 105 ± 7.6 × 105 | 0.0 ± 0.0 |
|  | T1 | 3.3 × 108 ± 7.7 × 107 | 4.3 × 107 ± 2.5 × 107 | 1.3 × 106 ± 1.2 × 106 | 3.2 × 103 ± 2.7 × 103 |
11 | T0 | 4.1 × 106 ± 7.5 × 105 | 1.2 × 106 ± 2.5 × 105 | 5.1 × 105 ± 4.1 × 105 | 6.0 × 102 ± 3.8 × 102 |
|  | T1 | 3.4 × 107 ± 6.2 × 105 | 3.1 × 107 ± 1.0 × 107 | 7.8 × 105 ± 7.7 × 105 | 1.7 × 104 ± 3.1 × 103 |
12 | T0 | 3.4 × 105 ± 7.6 × 104 | 7.5 × 102 ± 3.0 × 101 | 1.7 × 107 ± 1.1 × 107 | 0.0 ± 0.0 |
|  | T1 | 1.3 × 106 ± 7.0 × 105 | 2.0 × 105 ± 9.3 × 104 | 5.8 × 105 ± 5.6 × 105 | 3.6 × 103 ± 6.4 × 102 |
13 | T0 | 3.5 × 107 ± 1.6 × 106 | 1.2 × 107 ± 2.6 × 105 | 1.8 × 105 ± 1.0 × 105 | 0.0 ± 0.0 |
|  | T1 | 2.3 × 107 ± 3.8 × 106 | 4.6 × 106 ± 4.4 × 105 | 2.5 × 105 ± 1.8 × 105 | 1.8 × 102 ± 4.3 × 101 |
14 | T0 | 1.1 × 107 ± 6.9 × 105 | 2.3 × 106 ± 1.6 × 106 | 1.1 × 106 ± 1.8 × 105 | 0.0 ± 0.0 |
|  | T1 | 5.4 × 107 ± 1.7 × 107 | 1.0 × 107 ± 6.5 × 106 | 7.2 × 105 ± 6.4 × 105 | 3.0 × 102 ± 3.0 × 101 |
15 | T0 | 6.1 × 107 ± 7.4 × 106 | 1.7 × 107 ± 8.3 × 106 | 3.9 × 105 ± 2.9 × 105 | 1.8 × 101 ± 1.6 × 101 |
|  | T1 | 2.5 × 107 ± 5.3 × 106 | 1.0 × 107 ± 5.8 × 106 | 2.5 × 105 ± 2.2 × 105 | 3.2 × 102 ± 1.4 × 102 |
16 | T0 | 1.3 × 109 ± 4.5 × 108 | 4.0 × 107 ± 1.2 × 107 | 2.0 × 106 ± 1.1 × 106 | 0.0 ± 0.0 |
|  | T1 | 1.3 × 109 ± 2.0 × 108 | 2.2 × 107 ± 3.8 × 106 | 1.0 × 106 ± 8.2 × 105 | 8.3 × 102 ± 1.4 × 101 |
17 | T0 | 1.6 × 107 ± 1.6 × 106 | 5.0 × 106 ± 3.2 × 106 | 1.3 × 107 ± 2.9 × 106 | 1.3 × 102 ± 1.1 × 102 |
|  | T1 | 2.2 × 107 ± 1.9 × 106 | 4.0 × 106 ± 2.7 × 106 | 1.5 × 107 ± 2.0 × 105 | 6.6 × 102 ± 9.5 × 101 |
18 | T0 | 1.1 × 105 ± 3.1 × 106 | 1.4 × 103 ± 4.4 × 102 | 3.1 × 107 ± 2.7 × 107 | 0.0 ± 0.0 |
|  | T1 | 3.7 × 105 ± 8.9 × 104 | 1.7 × 105 ± 7.3 × 104 | 3.0 × 106 ± 1.2 × 106 | 6.5 × 102 ± 1.2 × 102 |
19 | T0 | 5.2 × 107 ± 1.7 × 107 | 4.3 × 105 ± 1.8 × 105 | 2.5 × 106 ± 1.9 × 106 | 0.0 ± 0.0 |
|  | T1 | 2.0 × 107 ± 8.0 × 106 | 1.5 × 105 ± 9.4 × 104 | 2.0 × 106 ± 1.5 × 106 | 0.0 ± 0.0 |
20 | T0 | 6.6 × 106 ± 5.2 × 106 | 4.4 × 106 ± 2.2 × 106 | 1.0 × 107 ± 8.4 × 106 | 1.8 × 103 ± 2.6 × 102 |
|  | T1 | 7.0 × 106 ± 3.3 × 105 | 5.5 × 106 ± 3.3 × 106 | 2.7 × 105 ± 2.6 × 105 | 0.0 ± 0.0 |