Table 1 Primers used in this study.
f | Primer sequence | Application | Reference |
|---|---|---|---|
PAL1 | 5'-ATGGAAATGCTGAAATTCGGC-3' | Amplification of the OprL gene | De Vos et al. 1997 |
PAL2 | 5'-CTTCTTCAGCTCGACGCGACG'-3 | Amplification of the OprL gene | De Vos et al. 1997 |
10514 | 5'-TGGTGGCCTCGAGCAAGAGAACGG-3' | RAPD analysis | Kersulyte et al. 1995 |
14306 | 5'-GGTTGGGTGAGAATTGC-3' | RAPD analysis | Kersulyte et al. 1995 |
pilA | 5'-ATG AAA GCT CAA AAA GGC TTT ACC TTG AT-3' | Identification of pilA | Kus et al. 2004 |
pilB | 5'-TCC AGC AGC ATC TTG TTG ACG AA-3' | Identification of pilA | Kus et al. 2004 |
pilB2 | 5'-TGT TCA GGT CGC AAT AGG C-3' | Identification of pilA | Kus et al. 2004 |
pilB3Rev | 5'-CGG AGA TGC CTA CAA AGA GC | Identification of pilA | This study |
nadCFor | 5'-CAG AAG TAC GCG GTC ACC TG | Identification of pilA | This study |
tRNAThr | 5'-CGA ATG AGC TGC TCT ACC GAC AGA GCT-3' | Identification of pilA | Kus et al. 2004 |
fliCFor | 5'-GGC CTG CAG ATC NCC AA | Identification of fliC | Winstanley et al. 1996 |
fliCRev | 5'-GGC AGC TGG TTN GCC TG | Identification of fliC | Winstanley et al. 1996 |
fliCRev2 | 5'-TTA GCGCAG CAG GCT CAG | Identification of fliC | This study |
fliCFor3 | 5'-ATG GCC TTG ACC GTC AAC ACC | cloning of fliC | This study |
fliCFor2 | -ATG GCC CTT ACA GTC AAC ACG | cloning of fliC | This study |
SeqU19 | 5'-GGT TTT CCC AGT CAC GAC G | sequencing of all cloned pilA and fliC | This study |
SeqT7 | 5'-CTA ATA CGA CTC ACT ATA GGG | sequencing of all cloned pilA and fliC | This study |
pre-pilA | 5'-GCG TTT GAA AGG TTG GCA TGC | sequencing of all cloned pilA | This study |
transrev | 5' CAG CAT AAC TGG ACT GAT TTC AG-3' | To check successful conjugation of the mini-Tn7 anneals to the inserted DNA | Koch et al. 2001 |
transfor | 5'-AAT CTG GCC AAG TCG GTG AC-3' | To check successful conjugation of the mini-Tn7, anneals to the 3'end of glmS | Koch et al. 2001 |