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Table 1 Primers used in this study.

From: Pseudomonas aeruginosa Cystic Fibrosis isolates of similar RAPD genotype exhibit diversity in biofilm forming ability in vitro

f Primer sequence Application Reference
PAL1 5'-ATGGAAATGCTGAAATTCGGC-3' Amplification of the OprL gene De Vos et al. 1997
PAL2 5'-CTTCTTCAGCTCGACGCGACG'-3 Amplification of the OprL gene De Vos et al. 1997
10514 5'-TGGTGGCCTCGAGCAAGAGAACGG-3' RAPD analysis Kersulyte et al. 1995
14306 5'-GGTTGGGTGAGAATTGC-3' RAPD analysis Kersulyte et al. 1995
pilA 5'-ATG AAA GCT CAA AAA GGC TTT ACC TTG AT-3' Identification of pilA Kus et al. 2004
pilB 5'-TCC AGC AGC ATC TTG TTG ACG AA-3' Identification of pilA Kus et al. 2004
pilB2 5'-TGT TCA GGT CGC AAT AGG C-3' Identification of pilA Kus et al. 2004
pilB3Rev 5'-CGG AGA TGC CTA CAA AGA GC Identification of pilA This study
nadCFor 5'-CAG AAG TAC GCG GTC ACC TG Identification of pilA This study
tRNAThr 5'-CGA ATG AGC TGC TCT ACC GAC AGA GCT-3' Identification of pilA Kus et al. 2004
fliCFor 5'-GGC CTG CAG ATC NCC AA Identification of fliC Winstanley et al. 1996
fliCRev 5'-GGC AGC TGG TTN GCC TG Identification of fliC Winstanley et al. 1996
fliCRev2 5'-TTA GCGCAG CAG GCT CAG Identification of fliC This study
fliCFor3 5'-ATG GCC TTG ACC GTC AAC ACC cloning of fliC This study
fliCFor2 -ATG GCC CTT ACA GTC AAC ACG cloning of fliC This study
SeqU19 5'-GGT TTT CCC AGT CAC GAC G sequencing of all cloned pilA and fliC This study
SeqT7 5'-CTA ATA CGA CTC ACT ATA GGG sequencing of all cloned pilA and fliC This study
pre-pilA 5'-GCG TTT GAA AGG TTG GCA TGC sequencing of all cloned pilA This study
transrev 5' CAG CAT AAC TGG ACT GAT TTC AG-3' To check successful conjugation of the mini-Tn7 anneals to the inserted DNA Koch et al. 2001
transfor 5'-AAT CTG GCC AAG TCG GTG AC-3' To check successful conjugation of the mini-Tn7, anneals to the 3'end of glmS Koch et al. 2001