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Table 1 Properties of genes that reduce the lethal effects of stress.

From: Escherichia coli genes that reduce the lethal effects of stress

Strain

MIC99 of Nal (μg/ml)a

Site of insertion

Functional annotation of disrupted genes

DM4100

4.5 ± 0.3

NA (wild-type)

NA

TL17

3.1 ± 0.1

yadC

Fimbrial-like protein

TL18

4.6 ± 0.3

ycdO

Putative lipoprotein

TL19

4.2 ± 0.6

yibA

Predicted lyase containing HEAT-repeat

TL20

4.6 ± 0.4

rfbX

RfbX lipopolysaccharide PST transporter

TL21

4.8 ± 0.2

rfbC

dTDP-4-deoxyrhamnose-3,5-epimerase

TL22

4.7 ± 0.1

ybdA

Permease (major facilitator superfamily (MFS) of transporters)

TL23

3.7 ± 0.3

yfbQ

Predicted aminotransferase

TL24

3.3 ± 0.2

ykfM

Predicted protein

TL25

3.0 ± 0.2

yrbB

Predicted NTP-binding protein

TL26

5.3 ± 0.3

ybcM

ARAC-type regulatory protein

TL28

3.4 ± 0.1

ycjW

Putative LACI-type transcriptional regulator

TL157

4.1 ± 0.5

ycjU

Putative β-phosphoglucomutase

TL158

4.0 ± 0.6

emrK

Putative membrane fusion protein

TL162

4.4 ± 0.6

emrY

Putative multidrug MFS transporter

  1. aMIC99 was measured by applying serial dilutions of mid-log phase cultures to agar plates containing various concentrations of nalidixic acid followed by incubation, colony number determination, and MIC99 estimation as described in Methods. The values shown are the means of 3 independent experiments with standard deviations as indicated. Abbreviations: Nal: nalidixic acid; NA: not applicable.