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Figure 6 | BMC Microbiology

Figure 6

From: Comparative proteomic analysis of Salmonella enterica serovar Typhimurium ppGpp-deficient mutant to identify a novel virulence protein required for intracellular survival in macrophages

Figure 6

STM3169 is regulated by ppGpp and ssrB. Transcriptional activity of stm3169 in Salmonella muntant strains. Salmonella Δrel A ΔspoT (A), ΔssrB (B), and ΔrelA ΔspoT ΔssrB (C) mutant strains carrying stm3196::lacZ fusion were incubated in MgM medium (pH5.8) for 18 h. The promoter activity of stm3169 was estimated by mesuring the β-garactosidase activity. L-arabinose (a final concentration of 0.001%) and IPTG (a final concentration of 0.01 mM) were added in the medium for induction of RelA on pRelA and for SsrB on pSsrB, respectively. Asterisks indicate that differences were statistically significant (P < 0.05).

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BMC Microbiology

ISSN: 1471-2180

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