Adhesion abilities of E. coli to HEp-2 cells. (A) Adhesion of FITC-conjugated ET2, and ET3 to HEp-2 cells. The adhesion ability is expressed as the ratio of florescence from adherent bacteria to that from inoculated bacteria. Bacteria were treated with proteinase K before FITC conjugation. Data represent means of five experiments with triplicate samples in each experiment. ET2, E. coli expressing vector only. ET3, E. coli expressing Scl1. (B) SDS-PAGE and western blot analysis of purified recombinant Scl1 protein. Lane 1 indicates the SDS-PAGE of purified rScl1. Lane 2 indicates the purified rScl1 protein confirmed by western blot analysis using anti-Scl1 antibody. rScl1 is indicated by a 48 kDa band. (C) Inhibition of binding by rScl1 protein and anti-Scl1 antibody. Prior to the adhesion assay, HEp-2 cells were pre-treated with rScl1 protein and ET3 were pre-treated with anti-Scl1 antibody and mouse IgG, respectively. **, P < 0.01 and ***, P < 0.001.