Table 3 Characteristics of VNTR markers for fingerprinting of Aspergillus fumigatus
From: Multiple-locus variable-number tandem repeat analysis for molecular typing of Aspergillus fumigatus
VNTR markers | Primer sequences (5' to 3') | Tm (°C) | Unit repeat size (bp) | Range of repeat number | Simpson diversity index* | Marker location (non coding region or name of gene if coding) |
|---|---|---|---|---|---|---|
Asp 167 | TGAGATGGTTAACTTACGTAGCGC CGCTCCCACCGTTACCAAC | 59 | 12 | 4-8 | 0.7151 | Chromosome 1 (GPI anchored serine-rich protein) |
Asp 202 | AGGATCACTGCCCTCAACCC CCGAAATCCGCGGGA | 59 | 12 | 6-14 | 0.8530 | Chromosome 1 (c-24(28) sterol reductase) |
Asp 330 | ATCTGGTCGCGAAATTCCTCT TCTTCGGCCTTTTCATCCC | 58 | 11 | 2-8 | 0.7895 | Chromosome 1 (non coding) |
Asp 443 | AAGCTTCGTCTGGCGAAGAG GCACGTGTACGGTGTTCCTG | 58 | 18 | 0-7 | 0.6661 | Chromosome 1 (ribosome assembly protein Noc2) |
Asp 446 | CGATCATGTTTGCCTGAGGA CCGACAGCATCGAGCAACTA | 59 | 21 | 1-4 | 0.5971 | Chromosome 1 (non coding) |
Asp 165 | TGATGGGCCGCAGTCG GCACCTGCTTGTCGATTCGT | 60 | 10 | 0-6 | 0.7296 | Chromosome 5 (non coding) |
Asp 252 | CAGATTGGAGACACGAAGCG ACCACGGATTGCCAAGGA | 58 | 12 | 2-6 | 0.5886 | Chromosome 5 (non coding) |
Asp 345 | TCTCCAACCCTTCGGACG GCCGGAAGAGCATGAAGACA | 58 | 11 | 1-6 | 0.5771 | Chromosome 5 (non coding) |
Asp 204 | GATGCGGGAGGTGGGTC CGTCCTCACTTTTGCCTTGG | 58 | 11 | 1-5 | 0.6128 | Chromosome 6 (non coding) |
Asp 20 | GGGAAGAGAGGAACCGATCC CGCAGTGGGCAGTTTGAAT | 58 | 10 | 0-4 | 0.7520 | Chromosome 8 (non coding) |