organism | Target | Efficiency (%) | Repeatability (SD of Cq)a | LOD target amplicons (copies/reaction)b | LOD gDNA (fg/reaction)b |
---|---|---|---|---|---|
B. anthracis | sspE | 94.5 | 0.045 | 2.6 (1.6-7.5) | 22.6 (9.9-148.5) |
 | cya | 94.7 | 0.057 | 6.5 (3.7-19.6) | 50.5 (19.1-408.3) |
 | capB | 94.8 | 0.051 | 3.6 (2.0-10.7) | 15.7 (9.9-78.9) |
F. tularensis | fopA | 98.2 | 0.042 | 7.2 (3.5-24.7) | 11.8 (5.5-66.4) |
 | ISFtu2 | 98.1 | 0.075 | 4.1 (2.2-12.8) | 0.6 (0.2-3.4) |
 | pdpD | 95.9 | 0.067 | 6.1 (3.1-20) | 4.2 (2.5-25.6) |
Y. pestis | ypo0393 | 93.1 | 0.057 | 1.7 (1.2-3.5) | 116 (59.3-967.2) |
 | caf1 | 93.2 | 0.099 | 1.9 (1.3-4.1) | 43.2 (23.9-277.2) |
 | pla | 93.1 | 0.047 | 3.6 (2.2-8.9) | 29.6 (13.5-191.9) |
B. thuringiensis | cry1 | 94.6/95/92.9c | 0.047/0.055/0.057 c | ND | ND |