Figure 5

Late stage P1 pupa. Overview (A) and detailed images of different optical sections (B - E) of the midgut of a C. floridanus pupa (P1) at a later stage than the pupa shown in Fig. 4 by confocal laser scanning microscopy (for further information regarding the composition of the figure see legend of Fig. 1). The bacteriocyte layer encloses the entire midgut (C) and the infection of midgut cells other than bacteriocytes (i.e. cells with large and nucleoli-rich nuclei) is increasingly observed (white arrows in D, E). Bacteria-harboring cells are now found in the epithelial layer bordering the gut lumen (E). Green label: The Blochmannia specific probe Bfl172-FITC; red label: SYTO Orange 83. The scale bars correspond to 220 μM (A) and 35 μM (B - E), respectively.