Table 1 Bacterial strains and plasmids used in this study.

V. cholerae

N16961

O1 El Tor, VPI-2 +, Sm^R

[57]

RAM-1

N16961, ΔVC1758, Sm^R

[23]

SAM-1

RAM-1, pIntV2, Sm^R Cm^R

This study

SAM-3

N16961, ΔVC1785, Sm^R

This study

SAM-4

N16961, ΔVC1809, Sm^R

This study

SAM-5

SAM3, pVefA, Sm^R Cm^R

This study

SAM-11

N16961, pBAD33, Sm^R Cm^R

This study

SAM-12

RAM-1, pBAD33, Sm^R Cm^R

This study

SAM-13

SAM-3, pBAD33, Sm^R Cm^R

This study

Plasmids

pDS132

Suicide plasmid, Cm^R, SacB

[59]

pBAD33

Expression plasmid, Ara, Cm^R

[60]

pIntV2

vc1758 cloned into pBAD33

This study

pD1785

ΔVC1785 cloned into pDS132

This study

pD1809

ΔVC1809 cloned into pDS132

This study

pVefA

vc1785 cloned into pBAD33

This study