Silver-stained SDS-PAGE gel of the LOS extracted from C. jejuni NCTC 11168 and 520. (a) Controls of M. catarrhalis serotype A (strain 2951) LOS for relative sizing LOS. Lanes: 1, M. catarrhalis wild-type LOS (WT); 2, M. catarrhalis M¯mutant LOS lacking the terminal glucose; (b) C. jejuni 11168-O and 11168-GS LOS extracted from bacteria grown at 37°C and 42°C. Lanes: 3, 11168-O at 37°C; 4, 11168-O at 42°C; 5, 11168-GS at 37°C; 6, 11168-GS at 42°C. (b) C. jejuni 520 LOS extracts from bacteria grown at 37°C and 42°C. Lanes: 1, 520 at 37°C; 2, 520 at 42°C. Higher-Mr LOS resolved at ~6 kDa and lower-Mr LOS at ~4 kDa.