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Table 1 Percentage inhibition of protein synthesis in Vero cells upon addition of varying volumes of concentrated culture supernatants.

From: Bacillus cereus cytotoxins Hbl, Nhe and CytK are secreted via the Sec translocation pathway

Strains and samples Supernatant
concentration factor
Amount of added concentrated
supernatant
Volume for
50%
inhibition*
   0.3 μl 1 μl 3 μl 10 μl 30 μl 100 μl  
ATCC 14579 without azide 40-fold -4% 21% 37% 89%    4.0 μl
ATCC 14579 with azide 40-fold    -7% 9% 70% 100% 20 μl
ATCC 14579 ten-fold -2% 50% 97% 100%    1.0 μl
ATCC 14579 ΔtatAC ten-fold 2% 45% 99% 100%    1.1 μl
ATCC 14579 ΔcomGA ten-fold -5% 49% 99% 100%    1.0 μl
Bt407 [plcA'Z] ten-fold -2% 44% 90% 100%    1.2 μl
Bt407 [plcA'Z] ΔflhA ten-fold    16% 72% 100% 100% 6.0 μl
  1. *Amount of supernatant required for 50% inhibition of protein synthesis (measured by C14-leucine incorporation) in Vero cells [35].