Table 1 Percentage inhibition of protein synthesis in Vero cells upon addition of varying volumes of concentrated culture supernatants.
From: Bacillus cereus cytotoxins Hbl, Nhe and CytK are secreted via the Sec translocation pathway
Strains and samples | Supernatant concentration factor | Amount of added concentrated supernatant | Volume for 50% inhibition* | |||||
|---|---|---|---|---|---|---|---|---|
0.3 μl | 1 μl | 3 μl | 10 μl | 30 μl | 100 μl | |||
ATCC 14579 without azide | 40-fold | -4% | 21% | 37% | 89% | 4.0 μl | ||
ATCC 14579 with azide | 40-fold | -7% | 9% | 70% | 100% | 20 μl | ||
ATCC 14579 | ten-fold | -2% | 50% | 97% | 100% | 1.0 μl | ||
ATCC 14579 ΔtatAC | ten-fold | 2% | 45% | 99% | 100% | 1.1 μl | ||
ATCC 14579 ΔcomGA | ten-fold | -5% | 49% | 99% | 100% | 1.0 μl | ||
Bt407 [plcA'Z] | ten-fold | -2% | 44% | 90% | 100% | 1.2 μl | ||
Bt407 [plcA'Z] ΔflhA | ten-fold | 16% | 72% | 100% | 100% | 6.0 μl | ||