Computational prediction of the Crc regulon identifies genus-wide and species-specific targets of catabolite repression control in Pseudomonas bacteria

Table 3 Comparison of predicted Crc regulon of P. aeruginosa with proteome data.

Gene name	PAO1	Function	protein
	PA0534	conserved hypothetical protein	2.03
hpd	PA0865	4-hydroxyphenylpyruvate dioxygenase	4.71
oprD	PA0958	Basic amino acid, basic peptide and imipenem outer membrane porin OprD precursor	1.75
	PA1069	hypothetical protein	4.28
	PA2553^a	probable acyl-CoA thiolase	1.59
	PA2555	probable AMP-binding enzyme	1.54
	PA2776	conserved hypothetical protein	1.71
	PA3187^b	probable ATP-binding component of ABC transporter	10.28
edd	PA3194	phosphogluconate dehydratase	2.17
	PA4500	probable binding protein component of ABC transporter	3.48
	PA4502^c	probable binding protein component of ABC transporter	3.35
	PA4506^c	probable ATP-binding component of ABC dipeptide transporter	8.43
dadA	PA5304	D-amino acid dehydrogenase, small subunit	2.36

Genes differentially regulated, based on proteome data, in rich media in a crc mutant of P. aeruginosa PAO1 [27] are cross referenced with predicted targets from all P. aeruginosa strains considered in this study. Values of protein indicate relative levels of protein in the crc mutant relative to levels in the wild type strain. Some genes are proximal to, and possibly in operons with, bioinformatically predicted Crc targets: (a) PA2553 is proximal to PA2555, (b) PA3187 is proximal to PA3186 and (c) PA4502 and PA4506 are proximal to PA4501.

ISSN: 1471-2180