Development of Loop-Mediated Isothermal Amplification (LAMP) Assays for Rapid Detection of Ehrlichia ruminantium

Table 1 Specificities of pCS20 PCR, pCS20 real-time PCR, pCS20 LAMP, and sodB LAMP assays

Rickettsial bacteria	Isolate	Origin	pCS20 PCR	pCS20 real-time PCR	pCS20 LAMP	*sodB* LAMP
Ehrlichia ruminantium	Ball 3	South Africa	+	+	+	+
	Burkina Faso	Burkina Faso	+	+	+	+
	Crystal Springs	Zimbabwe	+	+	+	+
	Gardel	Guadelope, Caribbean	+	+	+	+
	attenuated Gardel	Guadelope, Caribbean	+	+	+	+
	Ifé Nigeria	Nigeria	+	+	+	+
	Kerr Seringe	Gambia	+	+	+	+
	Kiswani	Kenya	+	+	+	+
	Kwanyanga	South Africa	+	+	+	+
	Lutale	Zambia	+	+	+	+
	Pokoase 471	Ghana	+	+	+	+
	Sankat 430	Ghana	+	+	+	+
	São Tomé	São Tomé and Principe	+	+	+	+
	Senegal	Senegal	+	+	+	+
	attenuated Senegal	Senegal	+	+	+	+
	Um Banein	Sudan	+	+	+	+
	Welgevonden	South Africa	+	+	+	+
	Zeerust	South Africa	+	+	+	+
Ehrlichia canis			+	-	-	-
Ehrlichia chaffeensis			+	-	-	-
Anaplasma centrale			-	-	-	-
Anaplasma marginale			-	-	-	-
Anaplasma phagocytophilum			-	-	-	-

ISSN: 1471-2180