Figure 4

Mutants that fail to produce MglA display increased transcript levels relative to the WT. cDNA was produced from mRNA harvested from the WT, ΔmglBA mutant and complementing strains as described in Methods and analyzed by qRT-PCR (Applied Biosystems). Background fluorescence was subtracted using the no-template control (NTC), resulting in the data shown. The data (n = 6) shown are relative to the normalized WT (value = 1). In order, the bars represent the WT, DK6204, MxH2432 (T78D) and MxH2406 (T54A) as positive controls as mutants that make MglA in amounts detectable by Western blot, and the mutants G19A, K25A, T26N, Q82A, Q82R, L117/120A, N141A, K142A and D144A respectively. MglA: (+) = made MglA, (--) = did not make MglA.