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Table 1 Determination of mean biofilm thickness and mean dimension of the zone in which GFP was expressed.

From: Physiology of Pseudomonas aeruginosa in biofilms as revealed by transcriptome analysis

Strain (plasmid) IPTG (mM) Biofilm*† Thickness (μm ± SD) GFP zone*† dimension (μm ± SD) Maximum† Fluorescence intensity (arbitrary ± SD)
PAO1 (pAB1) 0 165 ± 100 none 24 ± 26
PAO1 (pAB1) 1 170 ± 78 66 ± 30 166 ± 61
PAO1 (pMF54) 1 120 ± 38 none 3 ± 1
  1. *The thickness of the area of GFP expression as well as the overall thickness of the biofilm was measured 3 times. Measurement of Pseudomonas aeruginosa PAO1 carrying plasmid pAB1 containing an IPTG-inducible GFP with and without IPTG are compared with P. aeruginosa carrying plasmid pMF54 lacking GFP.
  2. †The uncertainties indicated are standard deviations.