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Table 1 Determination of mean biofilm thickness and mean dimension of the zone in which GFP was expressed.

From: Physiology of Pseudomonas aeruginosa in biofilms as revealed by transcriptome analysis

Strain (plasmid)

IPTG (mM)

Biofilm*† Thickness (μm ± SD)

GFP zone*† dimension (μm ± SD)

Maximum† Fluorescence intensity (arbitrary ± SD)

PAO1 (pAB1)

0

165 ± 100

none

24 ± 26

PAO1 (pAB1)

1

170 ± 78

66 ± 30

166 ± 61

PAO1 (pMF54)

1

120 ± 38

none

3 ± 1

  1. *The thickness of the area of GFP expression as well as the overall thickness of the biofilm was measured 3 times. Measurement of Pseudomonas aeruginosa PAO1 carrying plasmid pAB1 containing an IPTG-inducible GFP with and without IPTG are compared with P. aeruginosa carrying plasmid pMF54 lacking GFP.
  2. †The uncertainties indicated are standard deviations.