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Table 1 Strains and plasmids used in this study

From: Pellicle formation in Shewanella oneidensis

Strain or plasmid Relevant genotype Reference or source
E. coli   
   WM3064 Donor strain for conjugation; ΔdapA [53]
S. oneidensis   
   MR-1 Wild-type ATCC 700550
   JZ3253 flgA deletion mutant derived from MR-1; Δ flgA This study
   JZ4320 aggA deletion mutant derived from MR-1; ΔaggA This study
Plasmid   
   pDS3.0 Apr, Gmr, derivative from suicide vector pCVD442 Lab stock
   pBBR1MCS-5 Gmr vector used for complementation Lab Stock
   pDS-AGGA aggA deletion construct in pDS3.0 This study
   pDS-FLGA flgA deletion construct in pDS3.0 This study
   pBBR-AGGA pBBR1MCS-5 containing aggA of S. oneidensis This study
   pBBR-FLGA pBBR1MCS-5 containing flgA of S. oneidensis This study