Table 1 Strains and plasmids used in this study
Strain or plasmid | Relevant genotype | Reference or source |
|---|---|---|
E. coli | Â | Â |
   WM3064 | Donor strain for conjugation; ΔdapA | [53] |
S. oneidensis | Â | Â |
   MR-1 | Wild-type | ATCC 700550 |
   JZ3253 | flgA deletion mutant derived from MR-1; Δ flgA | This study |
   JZ4320 | aggA deletion mutant derived from MR-1; ΔaggA | This study |
Plasmid | Â | Â |
   pDS3.0 | Apr, Gmr, derivative from suicide vector pCVD442 | Lab stock |
   pBBR1MCS-5 | Gmr vector used for complementation | Lab Stock |
   pDS-AGGA | aggA deletion construct in pDS3.0 | This study |
   pDS-FLGA | flgA deletion construct in pDS3.0 | This study |
   pBBR-AGGA | pBBR1MCS-5 containing aggA of S. oneidensis | This study |
   pBBR-FLGA | pBBR1MCS-5 containing flgA of S. oneidensis | This study |