Figure 3

Treatment of S. oneidensis pellicles with EDTA and divalent cations. (A) Pellicle formation of the WT after 48 h in static LB in the presence of 0.3 mM EDTA and certain divalent cation (0.3 mM) under aerobic conditions. (B) Cells in pellicles formed in the presence of 0 (light blue), 0.1 (dark red), 0.2 (light yellow), and 0.3 mM (dark blue) EDTA at the different time points. Presented are averages of four replicates with the standard deviation indicated by error bars. (C) Effects of divalent cations on the inhibition of pellicle formation by EDTA. Pellicle formation of the WT after 48 h in static LB in the presence of 0.3 mM EDTA and one of indicated divalent cations (0.3 mM) under aerobic conditions was shown. The WT in static LB without EDTA was used as the control. The relative pellicle formation ((EDTA and indicated cation)/EDTA-absence control) was presented in the figure. EDTA only ('No cation' was used as the negative control. Presented are averages of four replicates with the standard deviation indicated by error bars.