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Table 1 Bacterial strains and plasmids

From: The role of glyceraldehyde 3-phosphate dehydrogenase (GapA-1) in Neisseria meningitidis adherence to human cells

Strain or plasmid Description Source or reference
Strains   
   E. coli   
JM109 Cloning strain Promega, Madison, WI
TOP10F' Cloning strain Invitrogen, Carlsbad, CA
BL21(DE3)pLysS Expression strain Invitrogen, Carlsbad, CA
   N. meningitidis   
MC58 wild-type serogroup B strain [26]
MC58ΔgapA-1 gapA-1 deletion and replacement with kanamycin cassette This study
MC58ΔgapA-1 gapA-1Ect MC58ΔgapA-1 complemented with an ectopic copy of gapA-1 This study
MC58ΔsiaD siaD deletion and replacement with erythromycin cassette C. Tang Imperial College
MC58ΔsiaD ΔgapA-1 siaD and gapA-1 deficient strain generated from MC58ΔsiaD using pSAT-8 This study
Plasmids   
pCRT7/NT-TOPO Cloning vector encoding resistance to ampicillin Invitrogen, Carlsbad, CA
pDT-GapA1 MC58 gapA-1 gene cloned in pCRT7-TOPO This study
pGEM-T Easy Cloning vector encoding resistance to ampicillin Promega, Madison, WI
pSAT-6 3-kb fragment spanning the MC58 gapA-1 region cloned in pGEM-T Easy This study
pJMK30 Source of kanamycin resistance cassette [43]
pSAT-8 pSAT-6 containing the kanamycin resistance cassette in the same orientation as the deleted gapA-1 gene This study
pSAT-12 Complementation vector containing cbbA and encoding resistance to erythromycin [29]
pSAT-14 pSAT-12 containing gapA-1 in place of the deleted cbbA This study