The role of T3SS in plant infection. (A) Cytotoxicity of wild-type B. pseudomallei and its T3SS mutants on THP-1 cells infected for six hours at an MOI of 100:1. Growth of B. pseudomallei and its T3SS mutants in LB (B) and MS (C) media. The graph is representative of two separate experiments. (D) Virulence of wildtype B. pseudomallei and its T3SS mutants on tomato plantlets. The average disease score with standard deviation is calculated based on at least 100 plantlets cumulative from several experiments.