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Table 3 Bacterial strains and plasmids used in the present study

From: A rapid and simple method for constructing stable mutants of Acinetobacter baumannii

Strain or plasmid Relevant feature(s) Source or reference
Strains   
   Acinetobacter baumannii   
ATCC 17978 Wild-type strain Laboratory stock
omp33::TOPO Derived from ATCC 17978. omp33 mutant obtained by plasmid insertion. KanR, ZeoR Present study
Δomp33::Km Derived from ATCC 17978. omp33 mutant obtained by gene replacement. KanR Present study
ΔoxyR::Km Derived from ATCC 17978. oxyR mutant obtained by gene replacement. KanR Present study
ΔsoxR::Km Derived from ATCC 17978. soxR mutant obtained by gene replacement. KanR Present study
ΔoxyR::Km-omp33::TOPO Derived from ΔoxyR::Km. oxyR omp33 double mutant. KanR, ZeoR Present study
ΔsoxR::Km-omp33::TOPO Derived from ΔsoxR::Km. soxR omp33 double mutant. KanR, ZeoR Present study
   Escherichia coli   
TG1 supE thi-1 Δ(lac-pro AB) Δ(mcr B-hsd SM)5(rK- mK-) [F' traD 36 pro AB lac IqZΔM15] Laboratory stock
Plasmids   
   pCR-BluntII-TOPO Suicide plasmid for A. baumannii. KanR, ZeoR Invitrogen
   pTOPO33int pCR-BluntII-TOPO containing a 387-pb internal fragment of the omp33 gene. KanR, ZeoR Present study
   pET-RA A. baumannii replication origin. CTX-M14 β-lactamase gene promoter. RifR Present study
   pET-RA-OMP33 pET-RA containing the omp33 gene without its promoter region. RifR Present study
  1. Kan, kanamycin; Zeo, zeocin; Rif, rifampicin