Target | Location | Primer sequence |
---|---|---|
Cm cassette | pKD3 forward 1 | GTGTAGGCTGGAGCTGCTTC |
 | pKD3 reverse 1 | CATATGAATATCCTCCTTA |
 | pKD3 forward 2 | ACCTGTGACGGAAGATCACTTCG |
 | pKD3 reverse 2 | AGGAACTTCATTTAAATGGCGCG |
flanking sequence of VP0220 (wbfF) gene | upstream forward* | CCCAGCCATAACTAACACTAACCCGT |
 | upstream reverse | GAAGCAGCTCCAGCCTACACGATAATTCGCTATTTAAATCGAGAGTTAAA |
 | downstream forward | TAAGGAGGATATTCATATGGGAACGACAAGATCATTCCAATCAG |
 | downstream reverse* | TAGGCTAAGTTCTGAGAGGTTTCCG |
flanking sequence of CPS region VP0219-0237 | upstream forward | AATACTAGTGAGCTGTGTTCTTCATTATTAATCCT |
 | upstream reverse | CGAAGTGATCTTCCGTCACAGGTGCAGTGAATGTCTGTTAACTCT |
 | downstream forward | CGCGCCATTTAAATGAAGTTCCTCAGGCTCGTTACCAATGTGCT |
 | downstream reverse | GCCAATTATCCTAGACTCACCACT |
flanking sequence of VP0215-0218 | upstream forward | CACCAGCATTGATCTGGTTATTCA |
 | upstream reverse | CGCGCCATTTAAATGAAGTTCCTTATTTAAGGGAGCTTCGGCTCCCT |
 | downstream forward | CGAAGTGATCTTCCGTCACAGGTAGAGCTGTGTTCTTCATTATTAATCC |
 | downstream reverse | TCGGCATTAGAGTAGCTCACTAACG |
flanking sequence of EPS genes VPA1403-1406 | upstream forward $ | CCACTACCCACAGAACCGCTTTGT |
 | upstream reverse | CGAAGTGATCTTCCGTCACAGGTATGACCTAGTTTCCCTTCTAGCA |
 | downstream forward | CGCGCCATTTAAATGAAGTTCCTAGCCAGGTTTAACCAACATATTGA |
 | downstream reverse$ | ACCACTCCAAAGGGTAGTGGTGA |
flanking sequence of wza, wzb, wzc genes | upstream forward | CAGGGAATCAAGCATACGTTGAA |
 | upstream reverse | CGAAGTGATCTTCCGTCACAGGTATTCATCTGACGTAAAGAGCGA |
 | downstream forward | CGCGCCATTTAAATGAAGTTCCTATCTAGATCGCTAATTTGACCAAA |
 | downstream reverse | GAGCAGCAAAGCTGCAGATTGA |