Skip to main content

Table 3 Features of PCR-amplified mycobacterial rhomboids

From: Rhomboid homologs in mycobacteria: insights from phylogeny and genomic analysis

  Primer Species/Strain Amplicon size (bp) ORF (bp) Amino acids Accession numberh Protein ID
Orthologs of Rv0110 (rhomboid protease 1) 0110F
0110R
aH37Rv 967 855 284 HM453890 ADO17908
   bBCG 967 855 284 HM453894 ADO17912
   cJN55 967 855 284 HM453896 ADO17914
   dBN44 967 855 284 HM453892 ADO17910
  5036F
5036R
eSMR5 1000 891 296 HM453900 ADO17919
Orthologs of Rv1337 (rhomboid protease 2) 1337F
1337R
H37Rv 869 723 240 HM453891 ADO17909
   BCG 869 723 240 HM453895 ADO17913
   JN55 869 723 240 HM453897 ADO17915
   BN44 869 723 240 HM453897 ADO17911
  1554F
1554R
fSU-36800 954 672 223 HM453898 ADO17916
   g27 954 291 (MAP2426c) 72 HM453899 ADO17917
     444 (MAP2425c) 147 HM453899 ADO17917
  4904F
4904R
SMR5 845 738 245 HM453901 ADO17920
  1. a: M. tuberculosis
  2. b: M. bovis
  3. c: M. bovis (cattle isolate)
  4. d: M. tuberculosis (patient isolate)
  5. e: M. smegmatis (streptomycin resistant derivative of MC2155)
  6. f: M. avium (patient isolate)
  7. g: M. avium subsp. Paratuberculosis (cattle isolate)
  8. h: Accession numbers are for GenBank
  9. Primer sequences are described in methods