Figure 4

PLD expressed inside HeLa cells is cytotoxic. HeLa cells were inoculated with A. haemolyticum strains and the bacteria were allowed to adhere for 2 h and invade for 5 h prior to determination of host cell viability. Viability is shown as a percentage of that of diluent-treated cells, which was set to 100%. Error bars indicate one standard deviation from the mean calculated from the averages of at least three independent experiments conducted in triplicate.