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Table 2 NTNH PCR detection in spiked healthy infant stool and a clinical infant botulism sample

From: Universal and specific quantitative detection of botulinum neurotoxin genes

Strain (genomic copies) NTNH Detection
BoNT A (104) ++
BoNT A (102) ++
BoNT A (10) +
BoNT A (1) +
BoNT B (104) ++
BoNT B (102) +
BoNT B (10) +
BoNT B (1) -
BoNT C (104) ++
BoNT C (102) +
BoNT C (10) +
BoNT C (1) -
BoNT D (104) ++
BoNT D (102) +
BoNT D (10) -
BoNT D (1) -
BoNT E (104) ++
BoNT E (102) +
BoNT E (10) +
BoNT E (1) -
BoNT F (104) ++
BoNT F (102) ++
BoNT F (10) +
BoNT F (1) -
BoNT G (104) ++
BoNT G (102) ++
BoNT G (10) +
BoNT G (1) -
Clinical stool sample ++
  1. ++ Denotes strong PCR band
  2. + Denotes weak PCR band
  3. - Denotes no PCR Band
  4. Listed in this table are results from NTNH gene detection within spiked samples of a healthy infant stool sample as described in the materials and methods. Also included is the result from a confirmed case of infant botulism in California. (++) indicates a strong positive PCR product at the dilution tested, (+) is a weak positive PCR product, and (-) indicates no amplification detected.