Figure 4
Changes in the T. pyriformis population in the co-culture with L. monocytogenes in dependence on L. monocytogenes LLO production. A. Detection of LLO in the culture supernatant of L. monocytogenes grown in the LB broth at 28°C. Proteins from 0,5 ml were loaded in each lane. On the left, secreted proteins are separated onto 10 % SDS-PAGE gel and visualized by staining with Coomassie Brilliant Blue R-250; on the right, Western blot analysis of secreted proteins with LLO-specific antiserum; 1 - wild type EGDe strain; 2 - EGDeΔhly strain carrying the vector pTRKL2; 3 - EGDeΔhly strain carrying the plasmid pHly. Numbers show molecular weight standard positions. The arrow shows a LLO position (MWLLO 58 kDa). B. Trophozoite (left) and cyst (right) concentrations related to LLO production: designations for columns are shown on the figure. The mean values ± SE from two experiments made in triplicate are shown. * p < 0,05.