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Figure 6 | BMC Microbiology

Figure 6

From: A high-throughput cloning system for reverse genetics in Trypanosoma cruzi

Figure 6

Schematic drawing showing the vector construction steps. The elements shown are the neomycin (NEO) and hygromycin (HYGRO) resistance genes, the T. cruzi intergenic region from ubiquitin locus (Tc UIR), the attachment sites for Gateway(r) recombination (att B1, att B2, att R1 and att R2), the chloramphenicol resistance gene (CmR), the gene for negative selection during cloning (ccd B), the fusion tags (6xhis, GFP, YFP, CFP, TAP and c-myc) and the ribosomal promoter (PR). In A, the steps for vectors construction are represented. In B, the vector reading frame with start and stop codons are shown.

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