C. salexigens CHR95 is not affected in the metabolism of ectoine. Cells grown in M63 with 1.5 M NaCl up to exponential phase were centrifuged, resuspended in the same medium to an OD600 of ca. 0.6, supplemented with 87 μM of [14C]-ectoine and incubated with and without 20 mM of glucose. After 2 h incubation at 37°C, CO2 production from ectoine (A) and macromolecules (EIF, B) and cytoplasmatic solutes (ESF, C) synthesized from ectoine, present in the ethanol insoluble and soluble fractions, respectively, were determined as described in Methods. The data are the averages of three different replicates ± SD (standard deviation).