Figure 2

C. salexigens CHR95 is affected in the transport and metabolism of glucose. Cells grown in M63 with 1.5 M NaCl up to exponential phase were centrifuged, resuspended in the same medium to an OD600 of c. 0.6, supplemented with 100 μM of [14C]-glucose. After different times of incubation at 37°C, the glucose remaining in the supernatant (S) and cytoplasmatic solutes synthesized from ectoine, present in the ethanol insoluble (EIF) and soluble (ESF) fractions, respectively, were determined as described in Methods. The data are the averages of three different replicates ± SD (standard deviation).