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Table 1 Plating efficiencies on SDS/EDTA

From: PpiD is a player in the network of periplasmic chaperones in Escherichia coli

Strain Plasmid Efficiency of platinga on 0.5 mM EDTA
   + 0.5% SDS + 2% SDS
wild-type None 0.90 0.54 ± 0.146
  pASK75 0.93 ± 0.061  
surA pASK75b 8.0, 0.028, and 0.011 [× 10-3]  
  pSurA 1.0 ± 0.13  
  pPpiDb 5.8, 0.011, and 0.032 [× 10-3]  
ppiD::Tn10    None   0.66 ± 0.156
  pASK75 0.96 ± 0.087  
ppiD::kan None   0.42 ± 0.184
  pASK75 0.81 ± 0.067  
surA ppiD::Tn10 pASK75b 2.6, 7.2, and 0.66 [× 10-3] .
  pSurA 0.7 ± 0.02  
  pPpiDb 4.1, 2.6, and 0.25 [× 10-3]  
Δskp None 0.87 ± 0.02 0.57 ± 0.042
  pQE60 1.04  
Δskp ppiD::kan none 1.01 ± 0.06 0.17 ± 0.042
  pQE60 1.0  
  1. aValues are the averages of at least three independent experiments.
  2. bThe plating efficiencies of three independent experiments are shown, as the wide range of variation did not allow averaging. Larger variations in the efficiencies of plating were observed only for strains showing strongly increased SDS/EDTA sensitivity and likely result from minor fluctuations in the concentration of these membrane perturbants in the different batches of medium (prepared freshly for each experiment).