Strain | Description | Reference |
---|---|---|
B. pseudomallei | Â | Â |
   DD503 | Parental strain; polymyxin BR zeocinS kanamycinS streptomycinR | [107] |
   DD503.boaA | Isogenic boaA mutant strain of DD503; polymyxin BR zeocinR kanamycinS streptomycinR | This study |
   DD503.boaB | Isogenic boaB mutant strain of DD503; polymyxin BR zeocinR kanamycinS streptomycinR | This study |
   DD503.boaA.boaB | Isogenic boaA boaB double mutant strain of DD503; polymyxin BR zeocinR kanamycinR streptomycinS | This study |
B. mallei | Â | Â |
   ATCC23344 | Wild-type strain; polymyxin BR zeocinS kanamycinS | [26] |
   ATCC23344.boaA | Isogenic boaA mutant strain of ATCC23344; polymyxin BR zeocinR kanamycinS | This study |
   E. coli |  |  |
   EPI300 | Cloning strain | EPICENTRE® Biotechnologies |
   S17 | Strain used for conjugational transfer of suicide plasmids from E. coli to B. pseudomallei or B. mallei | [108] |
Plasmids | Â | Â |
   pCC1™ | Cloning vector; chloramphenicol resistant (CmR) | EPICENTRE® Biotechnologies |
   pKAS46 | Mobilizable suicide plasmid; kanamycinR and ampicillinR | [109] |
   pCC1.3 | pCC1-based plasmid control, does not confer adherence; CmR | [102] |
   pSLboaA | pCC1 containing the B. mallei ATCC23344 boaA gene; CmR | This study |
   pSLboaAZEO | pSLboaA in which a zeocinR marker was introduced near the middle of the boaA gene; CmR and zeocinR | This study |
   pKASboaAZEO | pKAS46 containing the insert from pSLboaAZEO; zeocinR , ampicillinR and kanamycinR | This study |
   pSLboaB | pCC1 containing the B. pseudomallei DD503 boaB gene; CmR | This study |
   pSLboaBZEO | pSLboaB in which a zeocinR marker was introduced near the middle of the boaB gene; CmR and zeocinR | This study |
   pKASboaBZEO | pKAS46 containing the insert from pSLboaBZEO; zeocinR , ampicillinR and kanamycinR | This study |
   pKASboaB5' | pKAS46 containing a 0.8-kb insert which corresponds to a region located within the 5' end of the B. pseudomallei DD503 boaB ORF; ampicillinR and kanamycinR | This study |
   pKASboaB5'AmpS | pKASboaB5' in which the ampicillinR marker was removed; ampicillinS and kanamycinR | This study |
   pEM7ZEO | Source of the zeocinR marker; ampicillinR and zeocinR | Invitrogen™ |