Identification of Burkholderia mallei and Burkholderia pseudomallei adhesins for human respiratory epithelial cells

Balder, Rachel; Lipski, Serena; Lazarus, John J; Grose, William; Wooten, Ronald M; Hogan, Robert J; Woods, Donald E; Lafontaine, Eric R

doi:10.1186/1471-2180-10-250

Table 3 Strains and plasmids

From: Identification of Burkholderia mallei and Burkholderia pseudomallei adhesins for human respiratory epithelial cells

Strain	Description	Reference
B. pseudomallei
DD503	Parental strain; polymyxin B^R zeocin^S kanamycin^S streptomycin^R	[107]
DD503.boaA	Isogenic boaA mutant strain of DD503; polymyxin B^R zeocin^R kanamycin^S streptomycin^R	This study
DD503.boaB	Isogenic boaB mutant strain of DD503; polymyxin B^R zeocin^R kanamycin^S streptomycin^R	This study
DD503.boaA.boaB	Isogenic boaA boaB double mutant strain of DD503; polymyxin B^R zeocin^R kanamycin^R streptomycin^S	This study
B. mallei
ATCC23344	Wild-type strain; polymyxin B^R zeocin^S kanamycin^S	[26]
ATCC23344.boaA	Isogenic boaA mutant strain of ATCC23344; polymyxin B^R zeocin^R kanamycin^S	This study
E. coli
EPI300	Cloning strain	EPICENTRE^® Biotechnologies
S17	Strain used for conjugational transfer of suicide plasmids from E. coli to B. pseudomallei or B. mallei	[108]
Plasmids
pCC1™	Cloning vector; chloramphenicol resistant (Cm^R)	EPICENTRE^® Biotechnologies
pKAS46	Mobilizable suicide plasmid; kanamycin^R and ampicillin^R	[109]
pCC1.3	pCC1-based plasmid control, does not confer adherence; Cm^R	[102]
pSLboaA	pCC1 containing the B. mallei ATCC23344 boaA gene; Cm^R	This study
pSLboaAZEO	pSLboaA in which a zeocin^R marker was introduced near the middle of the boaA gene; Cm^R and zeocin^R	This study
pKASboaAZEO	pKAS46 containing the insert from pSLboaAZEO; zeocin^R , ampicillin^R and kanamycin^R	This study
pSLboaB	pCC1 containing the B. pseudomallei DD503 boaB gene; Cm^R	This study
pSLboaBZEO	pSLboaB in which a zeocin^R marker was introduced near the middle of the boaB gene; Cm^R and zeocin^R	This study
pKASboaBZEO	pKAS46 containing the insert from pSLboaBZEO; zeocin^R , ampicillin^R and kanamycin^R	This study
pKASboaB5'	pKAS46 containing a 0.8-kb insert which corresponds to a region located within the 5' end of the B. pseudomallei DD503 boaB ORF; ampicillin^R and kanamycin^R	This study
pKASboaB5'Amp^S	pKASboaB5' in which the ampicillin^R marker was removed; ampicillin^S and kanamycin^R	This study
pEM7ZEO	Source of the zeocin^R marker; ampicillin^R and zeocin^R	Invitrogen™

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ISSN: 1471-2180

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