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Table 3 Strains and plasmids

From: Identification of Burkholderia mallei and Burkholderia pseudomallei adhesins for human respiratory epithelial cells

Strain Description Reference
B. pseudomallei   
   DD503 Parental strain; polymyxin BR zeocinS kanamycinS streptomycinR [107]
   DD503.boaA Isogenic boaA mutant strain of DD503; polymyxin BR zeocinR kanamycinS streptomycinR This study
   DD503.boaB Isogenic boaB mutant strain of DD503; polymyxin BR zeocinR kanamycinS streptomycinR This study
   DD503.boaA.boaB Isogenic boaA boaB double mutant strain of DD503; polymyxin BR zeocinR kanamycinR streptomycinS This study
B. mallei   
   ATCC23344 Wild-type strain; polymyxin BR zeocinS kanamycinS [26]
   ATCC23344.boaA Isogenic boaA mutant strain of ATCC23344; polymyxin BR zeocinR kanamycinS This study
   E. coli   
   EPI300 Cloning strain EPICENTRE® Biotechnologies
   S17 Strain used for conjugational transfer of suicide plasmids from E. coli to B. pseudomallei or B. mallei [108]
Plasmids   
   pCC1™ Cloning vector; chloramphenicol resistant (CmR) EPICENTRE® Biotechnologies
   pKAS46 Mobilizable suicide plasmid; kanamycinR and ampicillinR [109]
   pCC1.3 pCC1-based plasmid control, does not confer adherence; CmR [102]
   pSLboaA pCC1 containing the B. mallei ATCC23344 boaA gene; CmR This study
   pSLboaAZEO pSLboaA in which a zeocinR marker was introduced near the middle of the boaA gene; CmR and zeocinR This study
   pKASboaAZEO pKAS46 containing the insert from pSLboaAZEO; zeocinR , ampicillinR and kanamycinR This study
   pSLboaB pCC1 containing the B. pseudomallei DD503 boaB gene; CmR This study
   pSLboaBZEO pSLboaB in which a zeocinR marker was introduced near the middle of the boaB gene; CmR and zeocinR This study
   pKASboaBZEO pKAS46 containing the insert from pSLboaBZEO; zeocinR , ampicillinR and kanamycinR This study
   pKASboaB5' pKAS46 containing a 0.8-kb insert which corresponds to a region located within the 5' end of the B. pseudomallei DD503 boaB ORF; ampicillinR and kanamycinR This study
   pKASboaB5'AmpS pKASboaB5' in which the ampicillinR marker was removed; ampicillinS and kanamycinR This study
   pEM7ZEO Source of the zeocinR marker; ampicillinR and zeocinR Invitrogen™