Characterization of the conserved sequence motif for MtrA in mycobacteria and C. glutamicum. (A) EMSA assays for validating the binding of MtrA with regulatory sequences of several potential target genes from M. tuberculosis. The promoter DNA of M. tuberculosis dnaA gene was used as positive control. An unrelated DNA was used as negative control. Several DNA substrates, namely, Rv0341_up, Rv0574c_up, and Rv3476c_up, were amplified from their promoter regions using specific primers. Several regulatory sequences of potential target genes from C. glutamicum including CglumepAp and CgluproPp, were amplified and used as DNA substrates. (B) A blast assay for the conserved sequence motif recognition by MtrA. Sequence alignment was carried and visualized by local BioEdit software. The complete consensus sequence is indicated by the stars under the base in the upper panel. Sequence logo was generated by WebLogo tool.