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Table 6 Strains and plasmids used in this study

From: Identification of the full set of Listeria monocytogenes penicillin-binding proteins and characterization of PBPD2 (Lmo2812)

Strain or plasmid Relevant genotype and features Reference or source
EGD L. monocytogenes wild-type  
KD2812 Δlmo2812 derivative of EGD This work
AD07 Δlmo2754 derivative of KD2812 This work
E. coli DH5α F- Φ80 Δ lac ZM15(lac ZYA-org F) U169 deo R rec A1 end A1 hsd R17 pho A sup E44kλ-thi-1 gyr A96 rel A1  
E. coli BL21(DE3) F- omp T gal dcm hsd SB(rB- mB-) λ(DE3) Novagen
pET30a   Novagen
pAD3 pET30a derivative containing lmo2812 gene This work
pKSV7 temperature-sensitive integration vector; MCSa; lac Z; β-lac; cat, pE194 Ts rep [31]
pKD2812 pKSV7 carrying the Δlmo2812 allele This work
pADPBP5 pKSV7 carrying the Δlmo2754 allele This work
  1. aMCS - multiple cloning site