Figure 4

HPLC elution patterns of muropeptides from wild-type and mutant L. monocytogenes peptidoglycan. Muropeptides produced by the enzymatic hydrolysis of peptidoglycan purified from wild-type L. monocytogenes EGD (A), mutant KD2812 lacking functional Lmo2812 (B), and mutant AD07 lacking functional Lmo2812 and PBP5 (C), were reduced and separated by reversed phase HPLC and the A₂₀₅ of the eluate was monitored: 1, 2 disaccharide-tripeptide monomers; 3,4,5 bis-disaccharide tri-tetra peptide dimers.