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Figure 6 | BMC Microbiology

Figure 6

From: The non-pathogenic mycobacteria M. smegmatis and M. fortuitum induce rapid host cell apoptosis via a caspase-3 and TNF dependent pathway

Figure 6

Macrophage apoptosis induction by a non-pathogenic mycoabcterium is caspase-3 and TNF-dependent. BMDMs from BALB/c mice were left untreated and uninfected (UT) or infected with M. smegmatis and then either left in medium (Msme) or treated with caspase-3 inhibitor (C3I), nonspecific chemical analog (C3I-A) neutralizing TNF antibody (TNF-Ab), nonspecific control Ab (Co-Ab) and TNF synthesis inhibitor pentoxifylline (PTX). The percentage of apoptotic cells out of 10,000 total cells was determined after 20 h using the hypodiploid PI flow cytometry assay and a representative histogram of two independent experiments performed in duplicates is shown.

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BMC Microbiology

ISSN: 1471-2180

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